A constant kLa ensures equal oxygen transfer independent of physical differences between each scale.A common scaling-up strategy for aerobic bioprocess is keeping the kLa constant within the different.kLa is a gas transfer coefficient, a measurement of the capacity of the bioreactor to transfer oxygen into.Oxygen plays an important role in aerobic bioprocesses and it is often the limiting factor of the bioreactor.M3, respectively in the 3 L and the 15 L those were set to 4.5 m/s and 9 KW/m3. To achieve this kLa value, the Vtip and Pg/VL for the 500 mL miniBio, were set to 2.6 m/s and 5.6 KW/ The kLa was fixed toĕ76 h-1 in the 3 bioreactors (maximum value measured for the 15 L bioreactor).(Table 2) that allows for the same kLa value in the 3 bioreactors. ![]() The correlations found in this study were used to determine the Vtip and Pg/VL for each bioreactor.Biomass was determined by measuring OD600 in a spectrophotometer and DCW at different time.The O2 and CO2 in the exhaust gas were measured with BlueSense off-gas sensors (BlueSense.The cultivations were controlled at 30 ☌ and pH 5 by automatic addition of 4 M KOH.(1992) (nicotinic acid was increased to 5 Yeast grew in minimal medium described by Verduyn et al.Was cultured in the 500 mL, 3 L and 15 L bioreactors. The yeast Kluyveromyces lactis (obtained from ATCC distributed by LGC standards, Wesel, Germany).The kLa was determined for the three bioreactors at varying aeration and agitation ranges as described.KLa is calculated by plotting the logarithmic change of oxygen concentration as a function of time. When liquid and gas phase are assumed ideally mixed, the Increase was registered as a function of time. The bioreactors were then sparged with air and the oxygen Nitrogen to strip oxygen from the bioreactors. The static method of gassing out was used to determine the kLa.ěriefly, demi-water was sparged with.This vessel has a ratio of impeller to reactor ofĐ.39 (Applikon The ratio of impeller to reactor diameter (D/Tv) is 0.35 for this vessel. ML except 3 baffles were added and the controller used was the ez-Control (Applikoněiotechnology,ĭelft). Theē L bioreactor had the same configuration as theĕ00 ![]() ![]() Parameters were controlled with the my-ControlĬontroller (Applikoněiotechnology,ĝelft). (D/Tv) (all material from Applikon Biotechnology, Delft). Sensor, L-type gas sparger, 2 rushton impellers, sampling pipe, 0.39 ratio impeller to reactor diameter The 500 mL bioreactor was assembled with an Applisens classic pH sensor, Lumisense optical DO.kLa was determined for a 500 mL miniBio, 3 L and 15 L bioreactors (Applikon Biotechnology, Delft).KLa as function of impeller tip speed and gassed power input over liquid volume The scalability is validated by applying the findings from the kLa study, to a K.Impeller tip speed (Vtip) or gassed power input of the stirrer per volume of liquid in the bioreactor (Pg/VL)Īre used to make kLa constant between bioreactors.The scale-up method of constant kLa is investigated.This work studies the scalability between 3 different volumes of lab scale bioreactors, 500 mL, 3 L and 15 L. ![]() F.1,2, Fernandes, C.2, D’Ambruoso, A.3, Koechlein, D.3ġDelft University of Technology, Van der MaasHZ Delft 2Applikon Biotechnology B.V., HeertjeslJG Delft, The Netherlands 3Applikon Biotechnology Inc. Scalability in Laboratory Bioreactors Based onĬonstant Volumetric Mass Transfer Coefficient (kLa)ĭe Jong, T. Table 1 | Aeration and agitation range used to determine kLa.īioreactor Aeration Agitation
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